Extended Data Fig. 5: Immunofluorescence labeling of arabinoxylan and cellulose in ZmGOLS2-VIGS and ZmGOLS2-OE maize stomata.
From: Esterified-pectin-coupled polar stiffening controls grass stomatal opening
a, Arabinoxylan labeling was weaker at the polar ends of ZmGOLS2-VIGS maize GCs. B73: n = 18 from 9 GCs; ZmGOLS2-VIGS: n = 43 from 22 GCs, **P = 0.0020. Student t-test. The experiment was repeated twice independently. b, Cellulose labeling was weaker at the polar ends of ZmGOLS2-VIGS maize GCs. B73: n = 20 from 10 GCs; ZmGOLS2-VIGS: n = 62 from 31 GCs, ****P = 7.43E-9. Student t-test. The experiment was repeated twice independently. c, Arabinoxylan labeling was stronger at the polar ends of ZmGOLS2-OE maize GCs. B104: n = 44 from 22 GCs; ZmGOLS2-OE: n = 50 from 25 GCs, **P = 0.0064. Student t-test. The experiment was repeated three times independently. d, Cellulose labeling was stronger at the polar ends of ZmGOLS2-OE maize stomata. B104: n = 34 from 17 GCs; ZmGOLS2-OE: n = 36 from 18 GCs, ****P = 1.17E-8. Student t-test. The experiment was repeated three times independently. e, De-methylesterfieid pectin was barely labelled in ZmGOLS2-VIGS, ZmGOLS2-OE and the wild-type maize plants. For each experiment mentioned above, three ZmGOLS2-VIGS plants and three plants of three ZmGOLS2-OE lines were used for collecting samples.
