Extended Data Fig. 4: ZmGOLS2-VIGS and ZmGOLS2-OE maize plants are similar in stomatal density, halftime of light-induced stomatal response and assimilation rate compared to the wild type.
From: Esterified-pectin-coupled polar stiffening controls grass stomatal opening
a, Construction of ZmGOLS2-VIGS lines and ZmGOLS2-OE lines. b, Positive control for ZmGOLS2-VIGS showing leaf bleaching by suppression of isopentenyl/dimethylallyl diphosphate synthase (ZmIspH) expression. c, Confirmation for transcription level of ZmGOLS2 by qRT-PCR. Values represent mean ± standard deviation, n = 3. **P =0.0064, 0.0062 and 0.0099 for VIGS-2, VIGS-3 and VIGS-5 versus B73; ***P = 1.69E-4, ****P =1.70E-5 and *P =0.0237 for OE-130, OE-132 and OE-134 versus B104. Two-sided student t-test. The experiments were repeated three times independently. d, Stomatal density of ZmGOLS2-VIGS and ZmGOLS2-OE maize plants. Values represent mean ± standard deviation, n = 15. P = 0.6194, P = 0.6391 and P >1.0000 for VIGS-2, VIGS-3 and VIGS-5 versus B73; P = 0.9821, 0.5870 and 0.0587 versus OE-130, OE-132 and OE-134 versus B104. Two-sided student t-test. The experiment was repeated twice independently. e, Halftime of light-induced stomatal response. n = 9. P = 0.4709 for ZmGOLS2-VIGS versus B73 for stomata close to open and P = 1.0000 for ZmGOLS2-VIGS versus B73 for stomata open to close. P = 0.6183, 0.5659 and 0.7676 for OE-130, OE-132 and OE-134 versus B104 for stomata close to open and P = 0.4216, 0.8617 and 0.1611 for OE-130, OE-132 and OE-134 versus B104 for stomata open to close. Two-sided student t-test. The experiment was repeated three times. f, Assimilation of ZmGOLS2-VIGS and ZmGOLS2-OE maize plants. Values represent mean ± standard deviation, n = 9. The experiment was repeated three times.
