Extended Data Fig. 8: Genes that vary along the CZE nodule-like-to-cyst trajectory.

a, Spearman correlation coefficients of the pairwise similarity between aggregated expression profiles of L3 CZE subtypes. 7 DAP L3 clusters could not be assigned apical/basal states. See Supplementary Table 3 to match abbreviated L3 names to their full descriptions. b, Left: L3 annotations for 3–5 DAP PEN and CZE clusters used in pseudotime analysis. Right: only blue-highlighted nuclei were used in the pseudotime expression plots in c to show gene expression variation in pseudotime along the non-basal cyst PEN to CZE trajectory (see Fig. 4). c, Normalized expression of a subset of genes that significantly vary on 3–5 DAP PEN/CZE pseudotime (see Fig. 4) is plotted in pseudotime-ordered nuclei. Color key in a extends to the labeling in c.