Extended Data Fig. 4: The stomatal development defect in epp1/5/6 is less severe than that in epp1/2/3/4/5/6.
From: Subtilase-mediated maturation of EPF1 and EPF2 is crucial for stomatal patterning
a–f, Representative confocal microscopy images of cotyledon abaxial epidermis from 5-day-old seedlings of wild type (a), epp1/2/3/4/5/6 (b), epp1/5/6 (c), epp1 (d), epp5 (e), and epp6 (f). Images were taken under the same magnification. Scale bar, 20 µm. g, Quantification of the stomatal number of indicated genotypes. h, Quantification of the stomatal index of indicated genotypes. For box plots (g, h), box limits indicate 25th and 75th percentiles; central lines indicate medians; whiskers display minimum and maximum values. Dots represent individual measurements per group. Different lowercase letters indicate statistically significant differences based on one-way ANOVA with Tukey’s multiple comparisons test (P < 0.05). The exact P values for all comparisons are provided in Supplementary Table 2. Sample sizes (number of biological replicates, each representing a cotyledon of an independent seedling): n = 15 (wild type), 10 (epp1/2/3/4/5/6), 11 (epp1/5/6), 10 (epp1), 14 (epp5), 13 (epp6).
