Extended Data Fig. 8: Treatment with MgRGF1 or AtRGF1 peptide activates MAPK cascade and PLT1/2 expression through RGI receptors.
a, b, MPK3 and MPK6 activation upon MgRGF1 or AtRGF1 peptide treatment. Wild-type seedings and the corresponding rgi mutants were tested in Arabidopsis (a) and rice (b). Five-day-old seedlings were treated with 10 μM sulfated peptides for the indicated times. Phosphorylated MPK3/6 was detected using an anti-pERK antibody. Equal loading was shown by anti-actin immunoblot. The experiments were performed three times with similar results. c, Peptide-induced promoter activities and protein levels of PLT1/2 were blocked in the Arabidopsis rgi mutant. Representative confocal images show root meristems of the transgenic seedlings expressing pPLT1::CFP, pPLT2::CFP, pPLT1::PLT1-YFP, or pPLT2::PLT2-YFP in wild-type and rgi mutant backgrounds. The experiments were performed three times with similar results. Scale bars, 100 μm.
