Extended Data Fig. 9: Sequence and expression analysis of PSY family genes in rice.
a, Multiple sequence alignment and Web-Logo analysis of rice PSYs. Conserved residues are highlighted in blue box. b, Gene expression levels of OsPSY genes at 1 dpi with M. graminicola. TPM values were retrieved from previously published RNA-seq data66. c, d, Relative expression analysis of OsPSY genes by RT-qPCR in Kitaake root tips after 3 days post M. graminicola inoculation (c) and 12 h MgRGF1-Y treatment (d). e, f, Relative expression levels of PSY family genes in root tips of Arabidopsis (e) and rice (f) after 24 h MgRGF1-Y treatment. Expression levels were quantified using the 2 − ΔΔCt method. AtActin2 and AtTUB2 served as internal controls for Arabidopsis, while OsUBQ10 and OsActin were used for rice. Data in c, d, e and f were presented as mean ± s.e.m.with individual data point of three independent biological replicates (n = 3). P values were determined by unpaired two-tailed Student’s t-test.
