Extended Data Fig. 1: MgRGF peptides promotes root development in Arabidopsis.
a, b, Root growth phenotype of Col-0 seedlings on vertical plates supplemented with or without 100 nM of the indicated peptides. Seedlings were photographed (a) and measured (b) at 7 days after germination (n = 15). Ctrl indicate no peptide added. Scale bars, 1 cm. c, d, Confocal images of Col-0 root tips stained with PI. Four-day-old seedlings were used for the staining (c). Number of meristematic cortex cells (d) was measured. n = 12, 11, 10, 11, 11 in d from left to right. Scale bars, 100 μm. e, Seven-day-old seedlings of Col-0 grown on 1.5% agar plates angled at 45°. Seedlings were grown vertically for 2 d on medium supplemented with the indicated peptides, then inclined for an additional 5 d. Representative images are shown, with at least 20 plants observed per treatment in each replicate. Scale bars, 1 cm. f-h, Root meristem phenotypes of tpst-1 grown in the absence or presence of the indicated peptides. Four-day-old seedlings were used for the PI staining and confocal imaging (f). Scale bars, 100 μm. Number of meristematic cortex cells (g) was measured (n = 10). Root length (h) of tpst-1 seedlings was measured at 7 days after germination (n = 15). i, j, Root meristem phenotypes of rgf1,2,3 grown in the absence or presence of indicated peptides. Confocal images showed root meristem of rgf1,2,3 stained with PI (i). Scale bars, 100 μm. Number of meristematic cortex cells (j) was measured (n = 15). Experiments were performed three times with similar results. White arrowheads mark the boundary between meristem and elongation zones and white asterisks indicate the QCs (c,f,i). Data in b, d, g, h and j are presented as box-whisker plots with individual data points. The central line indicates the median; box limits represent the first and third quartiles; whiskers extend to the minima and maxima. P values were determined by one-way ANOVA with Dunnett’s multiple comparisons test, comparing the mean of each column with the mean of the control column.
