Fig. 4
From: Conserved and species-specific molecular denominators in mammalian skeletal muscle aging
Aged skeletal muscle displays a decrease in OXPHOS complexes in primates. a Protein complexes from young (Y), middle-aged (M), and old (O) skeletal muscle from rhesus monkeys were isolated by blue-native electrophoresis (BNE) and stained with Coomassie (left panel) or in-gel complex I activity stain (right panel). Panels show a representative set of young, middle-aged, and old tissue out of 12 samples (four individuals in each age group). b Western blot detection of OXPHOS complexes and supercomplexes in 1D BN-blots using specific antibodies against complex I, II, III, IV, and V. c Densitometric quantification of in-gel activity stain (A, right panel) expressed as % of young complex I containing supercomplexes in four individuals each group. d Densitometric quantification of 1D BNE western blots. Signals of complexes and supercomplexes containing mitochondrial-encoded subunits were normalized to complex II not containing mitochondrial-encoded subunits (n = 4). e Protein complexes and subunit composition by 2D BN/SDS PAGE shown as silver stain from young (left panel), middle-aged (middle panel), and old (right panel). Assignment of complexes: S0, supercomplexes containing complex I and III with the stoichiometry of I1III2; S1, supercomplexes containing complex I, III, and IV with the stoichiometry of I1III2IV1; S, supercomplexes containing complex I, III, and IV with the stoichiometry of I1III2IV0–4; III2, dimer of complex III or cytochrome c reductase; IV, complex IV or cytochrome c oxidase; V, complex V or ATP synthase. f Skeletal muscle cardiolipin content (L4/L3O ratio) in subsarcolemmal (SS) and inter-myofibrillar (IMF) mitochondrial fraction from young (Y), middle-aged (M) and old rhesus monkeys. L4: tetra linoleoyl-cardiolipin, L3O: trilinoleoyl-oleoyl-cardiolipin. *p < 0.05 vs. young. Values are expressed as mean ± SEM
