Fig. 4: Nitrosative stress test on glycated Hb in RBCs.
From: Molecular phenotyping of oxidative stress in diabetes mellitus with point-of-care NMR system
a The A-ratio plot for RBCs in baseline (black) and pretreated sodium nitrite (red, green, blue) for subjects with non-DM subjects (n = 24), and DM subjects with their glycemic index in good glycemic control (n = 29), and (iii) poor glycemic control (n = 43) subgroups. The means and changes in each subgroups were indicated above the box-plots. The data is represented by box-plot format. b A quadrant chart of diabetic subjects stratified into subgroups based on their normalized oxidative status (nitrosative stress) in association with their glycemic levels (e.g., HbA1c). The threshold of Anitrosative-ratio was at 75th percentile that of DM subjects with good glycemic control, and comes with the flexibility of adjusting based on clinical needs (e.g., population genetics). Note that the Y-axis (nitrosative stress) is analogous with Y-axis (peroxidative stress) in the quadrant shown in Fig. 6b. The details of quadrant representations are shown in Fig. 1e. The statistical significance was calculated using the Student’s T-test (two-tailed, unequal variance). The data is represented by box-plot format. c The diagnostic accuracies were evaluated using receiver operating characteristic (ROC) for non-DM subjects against DM subjects with good glycemic control (blue) and poor glycemic control (red) subgroups. The number of subjects (n) is indicated in parentheses.
