Fig. 1: Schematic summary of the methodology. | npj Aging and Mechanisms of Disease

Fig. 1: Schematic summary of the methodology.

From: Differential transcript usage unravels gene expression alterations in Alzheimer’s disease human brains

Fig. 1: Schematic summary of the methodology.The alternative text for this image may have been generated using AI.

A Datasets obtained from three consortia (Mayo, MSBB, and ROSMAP) were grouped according to the brain region sampled in the frontal lobe (FL) or temporal lobe (TL). Next, RNAseq data were pseudo-aligned using Kallisto. Clinico-pathological classifications were included as metada. B scRNAseq data from the midle temporal gyrus (MTG, Allen Brain Atlas) were analyzed using the R package SEURAT. C Gene expression analyses were performed using the R packages DESeq2, IsoformSwitchAnalyzeR (ISAR), and gene set enrichment analysis (GSEA). Assignment of differentially expressed genes or isoform switches to specific cell types/subtypes was performed indirectly using scRNAseq signatures obtained from the MTG (B).

Back to article page