Fig. 1: Age and sex-associated differences in arginase-II protein expression.

a Immunoblotting analyses of arginase-II in kidney of male and female WT mice. Two scanning intensities are shown to better visualize the aging- and gender-associated differences in Arg-II levels (low intensity image was used for female mice and higher intensity image was used for male mice quantification). Kidney from arg-II^−/− mice was used as a negative control. Ponceau staining serves as loading control, since actin has a variable expression with aging. b Quantification is shown in the graphic revealing comparison of young and old mice. c Quantification is shown in the graphic revealing comparison of male and female mice. The value of male young and male old mice was used as a reference for female young and female old mice, respectively. Y-WT young WT, O-WT old WT, KO arg-II^−/−. ***p ≤ 0.001 between the indicated groups. Male: Y-WT, n = 6; O-WT, n = 11; female: Y-WT, n = 6; O-WT, n = 10.