Fig. 1: FKBP52 accelerates tau P301L protein aggregation. | npj Aging and Mechanisms of Disease

Fig. 1: FKBP52 accelerates tau P301L protein aggregation.

From: FKBP52 overexpression accelerates hippocampal-dependent memory impairments in a tau transgenic mouse model

Fig. 1

a Using a thioflavin T (ThT) fluorescence assay, tau P301L (20 μM) was monitored over 72 h with or without coincubation of FKBP52 (20, 2, or 0.8 µM). b Representative transmission electron microscopy (TEM) images are shown (×20,000 magnification; scale bar 1 μm). c Left panel: Representative dot blot analysis of tau aggregates incubated with 0.5 µg of tau P301L, 0.5 µg of FKBP52, or combination of (0.5 µg Tau and 0.5 µg FKBP52) probed with T22 antibody. Right panel: Quantification of T22 expression in dot blot with samples carried out in quadruplet. Results represented as standard error of the mean (±SEM); experiment was run in triplicate, **p < 0.001 by one-way ANOVA.

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