Fig. 2: Experimental design and confirmation of GFP and FKBP52 overexpression in the hippocampus. | npj Aging and Mechanisms of Disease

Fig. 2: Experimental design and confirmation of GFP and FKBP52 overexpression in the hippocampus.

From: FKBP52 overexpression accelerates hippocampal-dependent memory impairments in a tau transgenic mouse model

Fig. 2

a Timeline of bilateral hippocampal AAV injections and experiments performed in WT and rTg4510 mice. b Representative images of AAV9-GFP and AAV9-FKBP52 hippocampal sections after 3 months of viral expression. c Representative Western blots and quantification of GFP and FKBP52 overexpression in these animals. Wild-type mice [n = 16], rTg4510 mice [n = 16]. Protein levels were normalized to ß-Actin and analyzed by unpaired t test, where statistical significance is represented by **p < 0.01 and ***p < 0.001. Scale bar represents 200 µm; inset scale represents 20 µm. AAV9 adeno-associated virus serotype 9, GFP green fluorescent protein, WT wild-type, HPC hippocampus.

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