Fig. 2: Body composition and liver glycogen on day 5 of food restriction.

a Body composition in young and aged mice subjected to 5-day food restriction (FR). *P < 0.05, **P < 0.01 by Tukey−Kramer or Steel−Dwass test; young control (n = 6), young FR (n = 6), aged control (n = 5), and aged FR (n = 14). Tissue weights in young and aged mice subjected to 5-day FR. *P < 0.05, **P < 0.01, ***P < 0.001 by Tukey−Kramer or Steel−Dwass test. Liver, epididymal fat, and soleus; young control (n = 6), young FR (n = 8), aged control (n = 5), and aged FR (n = 12). Gastrocnemius: young control (n = 7), young FR (n = 8), aged control (n = 7), and aged FR (n = 15). b The hematoxylin and eosin staining of liver sections for liver glycogen content. Arrows indicate sites of glycogen accumulation. Bar = 50 µm. *P < 0.05, ***P < 0.001 by Tukey’s test; young control (n = 7), young FR (n = 8), aged control (n = 7), and aged FR (n = 15). Data are presented as mean ± SEM.