Table 1 List of primer sequences used for RT-qPCR analysis.
From: The killifish visual system as an in vivo model to study brain aging and rejuvenation
Gene | Primer sequence (5′> 3′) |
|---|---|
18s ribosomal RNA | F: ccgacattgacctcaacaa |
R: tggctgtatttgccatcc | |
Elongation factor 1α | F: actctggcattgtcgtttag |
R: agttaccagcagctttcttc | |
p21 | F: cgagccctcagtactctaa |
R: gaggtttgtcggagaagaag | |
p27 | F: gctcctcaatcgccatatt |
R: catacgtcgaagtggaagac | |
Tumor necrosis factor | F: caggctcacaagaggttatt |
R: ccagaggtcaatctgtcttatc | |
Sirtuin 1 | F: cgagccattctaagggattt |
R: ctgaccacatcttccaaagt | |
Interleukin 8 | F: acaaatcctgaccacaagtag |
R: atcgtattcaccatcatgtctc | |
Interleukin 6 | F: ggaggaatttcaagggaacata |
R: cctcaggagaaccatgtaga | |
Interleukin 10 | F: ccttacagcatcatgtctctc |
R: tggcagcagtcgtttatg | |
Interleukin 1β | F: ccgacagcaagaaacgaa |
R: gtggcaggacaggtataga |
