Fig. 7: Analysis of the effect of cell passage number, mitochondrial depletion, NLRP7 knockdown, continuous cellular reprogramming, and transient cellular reprogramming on predicted age comparing AltumAge and Horvath’s model.
From: A pan-tissue DNA-methylation epigenetic clock based on deep learning
a Scatter plot of predicted age of iPSCs and ESCs by passage number with best fit line with 95% confidence interval calculated from 1000 bootstrap iterations. b Box plots showing predicted age in 143B cells with mitochondrial depletion (rho0-) or control (rho+−). c Box plots showing predicted age of H9 ESCs with NLRP7 knockdown (NLRP7-) or control (Normal). d Time course analysis of OSKM reprogramming with standard deviation error bars. e Point plot showing age prediction in human fibroblasts and endothelial cells before and after transient reprogramming. The box and whiskers (b, c) show the quartiles of the data.
