Fig. 5: Assessment of the quantity and quality of ovulated oocytes after oral administration of single compounds in mice.

a Body weight changes and b ovary weight were assessed in five mice in each group following the administration of either 0.5% DMSO (vehicle) or 5 mg/kg of the compound for four weeks. The number of retrieved (c) total oocytes, (d) MII oocytes, and (e) MII oocytes with normal spindle alignment were counted to assess oocyte quantity and quality. Data are presented as the mean ± standard deviation. Statistical analyses were performed using a one-tailed unpaired t-test. p < 0.05. YC young control (12-week-old mice; sampling at this age), OC old control (42-week-old mice; sampling at this age), Veh vehicle, But butylphthalide, Ole oleanolic acid.