Fig. 2: Chronic administration of GinA and modGinA in aged C57BL/6JN mice.

A Study procedures for daily oral administration of GinA (10 mg/kg, n = 12) or modGinA (27 mg/kg, n = 14) or vehicle (high oleic acid sunflower oil, n = 12) in ~80 week old C57BL/6JN mice for 10 weeks. Blood was collected at 9 weeks during treatment and 10 days after termination of treatment, with brain, muscle and liver also collected. Behavioral studies were carried out following termination of treatment over a period of 10 days. B Circulating levels of IL-2, IL-5 and IL-6 during treatment (i) and after treatment (ii). Proteins level significantly different between treatment groups and vehicle are labeled (**p < 0.01; *0.01 < p < 0.05, Dunnetts test). C Total number of metabolites above the limit of detection (orange) in >70% of samples and total number of significant metabolites (blue) identified in serum, muscle, brain and liver using the unadjusted Kruskal–Wallis test. D Circulating levels of CE 22:6, CE 20:5 and choline in serum during treatment (9 weeks). Data were calculated using the unadjusted Kruskal–Wallis test. E Locomotor activity in an open field chamber was unaffected by treatment throughout the full 30 min of testing F Scatter-plot for all the behavioral tests carried out after termination of treatment and within a 10-day window post-treatment.