Fig. 2: Identification of bone marrow-derived MDSCs in aged human using MDSC signature genes. | npj Aging

Fig. 2: Identification of bone marrow-derived MDSCs in aged human using MDSC signature genes.

From: Defining the marker and developmental trajectory of myeloid-derived suppressor cells in aging by single-cell transcriptomics

Fig. 2: Identification of bone marrow-derived MDSCs in aged human using MDSC signature genes.

A Unbiased Seurat clustering analysis of bone marrow-derived neutrophils in the integrated scRNAseq dataset. The UMAP projection result showed eight distinct clusters in the neutrophils, both in male and female samples. B, C UMAP and violin plot results showed MDSC signature score ordered by neutrophil subset. Cluster 4 exhibited the highest expression of MDSC gene signature, both in male and female samples. D Heat map showing top 10 marker genes for each neutrophil subset. E Unbiased Seurat clustering analysis of bone marrow derived monocytes in the integrated scRNAseq dataset. The UMAP projection result showed five distinct clusters in the monocytes, both in male and female samples. F, G UMAP and violin plot results showed MDSC signature score ordered by monocyte subset. Cluster 0 exhibited the highest expression of MDSC gene signature, both in male and female samples. H Heat map showing top 10 marker genes for each monocyte subset. I MDSC signature score escalated with advancing age in neutrophils. J MDSC signature score escalated with advancing age in monocytes.

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