Fig. 3 | npj Biofilms and Microbiomes

Fig. 3

From: Ser/Thr protein kinase PrkC-mediated regulation of GroEL is critical for biofilm formation in Bacillus anthracis

Fig. 3

GroEL expression and phosphorylation in B. anthracis: a Immunoblots show the expression of GroEL in B. anthracis cell lysates. Equal amounts of lysates (5 µg each) were used and probed with anti-GroEL antibodies. Purified GST-GroEL (0.5 µg) was taken as the positive control. Left panel shows the comparative expression of GroEL in Bas-wt and BasΔprkC. Right panel shows the comparative expression of GroEL in Bas-wt and BasBF. b groEL was overexpressed and purified from B. anthracis strain, followed by immunoblotting with anti-pThr antibody. GroEL was phosphorylated in Bas-wt whereas no phosphorylation was observed with GroEL purified from BasΔprkC. c Two-dimensionally separated cell lysates of B. anthracis were probed with anti-GroEL antibodies to assess the stoichiometry of phosphorylation of native GroEL. Multiple species were observed in Bas-wt as compared with BasΔprkC, showing the in vivo phosphorylation of GroEL specifically by PrkC. GroEL was hyper-phosphorylated during biofilm formation (BasBF) as observed by the increased number of spots (third panel)

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