Fig. 7
Ratiometric imaging of O2 with oxygen-sensitive nanoparticles incorporated in alginate beads with P. aeruginosa. a Calibration of nanoparticles in alginate beads submerged in water of increasing O2 concentrations. The O2 was quantified as the ratio of the Red and Green channels extracted from RGB colour images obtained with a simple USB microscope. b Alginate bead with sensor nanoparticles, c zoom-in and visualisation of O2 distribution in alginate bead after 24 h of growth with P. aeruginosa. Size of bar: 200 µm
