Fig. 2
From: A microbiota-generated bile salt induces biofilm formation in Clostridium difficile
Characterization of the biofilm architecture and population in the presence of DOC. a CLSM images of C. difficile biofilms formed in the absence or presence of 240 µM DOC stained with Syto 9 (green/living) and propidium iodide (red/dead). b CLSM images of C. difficile grown in the absence or presence of 240 µM DOC and stained with FM4-64. a, b The CLSM analyses were performed with unwashed biofilms. c Cell length (µm) distribution within a population grown in BHISG or BHIG with 240 µM DOC. Asterisks indicate statistical significance determined with a two-tail Mann–Whitney test (****p ≤ 0.0001 vs BHISG without DOC)
