Table 1 Strains and plasmids used in this study
From: Colonization and immune modulation properties of Klebsiella pneumoniae biofilm-dispersed cells
Strain or plasmid | Description | Antibiotic resistances | Collection number | Source and/or reference |
|---|---|---|---|---|
Strains | ||||
K. pneumoniae | ||||
LM21-GFP-SpR | K. pneumoniae LM21 strain harboring the gfpmut3 and aadA7 genes | Sp | CH404 | |
Kp-ApR | K. pneumoniae clinicate isolate | Ap | CH1157 | This study |
Kp-SpR | Kp-ApR Δshv::aadA7 | Sp | CH1476 | This study |
Kp-GFP | mini-Tn7T-Km-GFPmut3 inserted into attTn7 sites of Kp-SpR | Sp, Km | CH1477 | This study |
Kp-mCherry | mini-Tn7T-Km-cCherry inserted into attTn7 sites of Kp-SpR | Sp, Km | CH1478 | This study |
E. coli K-12 | ||||
MFD λpir | MG1655 RP4-2-Tc::[∆Mu1::aac(3)IV-∆aphA-nic35-Mu2::zeo]∆dapA::(erm-pir) ∆recA | Ap, Zeo, Erm | ||
Plasmids | ||||
pTNS3 | Helper plasmid, providing the Tn7 transposition function. R6K ori, oriT | Ap | ||
pUC18R6KT-mini-Tn7T-Km | pUC18-based delivery plasmid for mini-Tn7-Km. R6K ori, oriT | Ap, Km | ||
pUC18R6KT-mini-Tn7T-Km-gfp | pUC18-based delivery plasmid for mini-Tn7-Km-gfp harboring GFP encoding gene. R6K ori, oriT | Ap, Km | This study | |
pUC18R6KT-mini- Tn7T-Km-mcherry | pUC18-based delivery plasmid for mini-Tn7-Km-mcherry harboring mCherry encoding gene. R6K ori, oriT | Ap, Km | This study | |
