Fig. 3

Metabolites associated with the urea cycle are significantly increased in RM33 biofilms. The concentrations of the metabolites associated with the urea cycle determined from information at KEGG and Biocyc were quantified as described in “Methods” section. The red X indicates absence of known orthologs of enzymes involved in the urea cycle for 86-028NP. The mean and s.e.m. of the concentrations for the metabolites from three independent biological replicates of 86-028NP (circle) and RM33 (square) 48 h biofilms are presented. Statistical significance was determined using a two-tailed unpaired Student’s t test (*p < 0.025)