Fig. 2
From: Rapid microevolution of biofilm cells in response to antibiotics
Results of the minimum inhibitory concentration (MIC) antibiotic susceptibility assay. X-axis: 30 planktonic isolates P101–P310 (P samples), 30 antibiotic-free biofilm effluent isolates B101–B310 (B samples), 30 ciprofloxacin-exposed biofilm effluent isolates C101–C310 (BC samples), and 30 tetracycline-exposed biofilm effluent isolates T101–T310 (BT samples). Each sample type includes 10 isolates from each biological replicate 1 (blue parentheses), biological replicate 2 (orange parentheses), and biological replicate 3 (gray parentheses). Blue bars represent the MIC levels of ciprofloxacin (in μg/ml, measured on the primary Y-axis on the left), and red bars—the MIC levels of tetracycline (in μg/ml, measured on the right-hand Y-axis). Consensus MIC levels in the initial planktonic cultures are shown by the horizontal blue (for ciprofloxacin MIC) and red (for tetracycline MIC) dashed lines. P values denote differences between sample pairs based on nested mixed factor ANOVA test followed by Turkey’s HSD post hoc test. P values showing statistically significant (p < 0.05) differences are presented in bold
