Fig. 1: Validation of variation of gene expression using RNA-seq and iTRAQ analyses.
From: Biofilm formation displays intrinsic offensive and defensive features of Bacillus cereus
a Fold-change in expression between transcriptomic (RNA-Seq) and proteomic (iTRAQ) results from samples at 24, 48, and 72 h. Points show significant changes (adjusted p-value < 0.05) according to both technologies (red), changes only found in proteomic (blue) or only found in transcriptomic (green). Gray dots represent items detected with statistically no significant changes. The top right and bottom left quadrants show concordant directions of change between both technologies; the top left and bottom right show conflicting directions. b Relative comparison of COG categories of genes and proteins. Total number of elements of each category were relativized to a percentage and distributed in down or upregulated. This graph shows that the ratio of elements that are up or downregulated within each COG category is conserved, revealing similar results in terms of functional categories among RNA-seq and iTRAQ techniques.
