Fig. 4: Motility of CIP-resistant colonies Plots representing the swimming (squares), swarming (triangles) and twitching (dots) motilities (median, and the ranges) in (mm) of CIP-resistant colonies isolated from evolution experiments under exposure to CIP (CIP) or without CIP exposure (CTRL).

For comparisons, the motilities of the WT PAO1 are presented by black symbols in the figure. A significant lower twitching motility (p = 0.03) and higher swarming motility (p = 0.0001) was observed in CIP-resistant colonies isolated from CIP compared to and CTRL biofilms. Mann–Whitney test was used for statistical analysis.