Fig. 3: SipW signal peptidase is necessary for the secretion and polymerization of BsaA proteins. | npj Biofilms and Microbiomes

Fig. 3: SipW signal peptidase is necessary for the secretion and polymerization of BsaA proteins.

From: Temperature-regulated heterogeneous extracellular matrix gene expression defines biofilm morphology in Clostridium perfringens

Fig. 3

a Western blotting of BsaA proteins. Cell extracts (C) and culture supernatants (S) were isolated from WT or sipW mutant cells harboring the indicated plasmids. Protein samples (OD600 = 0.01) were separated on a 4–12% gradient SDS-polyacrylamide gel. BsaA and SipW-FLAG proteins were detected with anti-BsaA and anti-FLAG antisera, respectively. b The SipW-FLAG-expressing plasmid restores pellicle biofilm formation of the sipW mutant. Cells were grown for 2 days at 25 °C. The edges of pellicle biofilms, formed at the bottom of the well, were picked by pipetting and photographed after gentle agitation.

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