Fig. 4: BsaA proteins form filamentous EPS and cover the surface of pellicle biofilms. | npj Biofilms and Microbiomes

Fig. 4: BsaA proteins form filamentous EPS and cover the surface of pellicle biofilms.

From: Temperature-regulated heterogeneous extracellular matrix gene expression defines biofilm morphology in Clostridium perfringens

Fig. 4

a SEM images of WT or bsaA mutant cultures. Cells were grown at 25 °C for 2 days. Bar = 2 μm. b CLSM images of cells stained with SYTO9 DNA dye (green) and anti-BsaA antibodies labeled with HiLyte Fluor 555 (red). Cells were grown at 25 °C for 2 days. Bar = 20 μm. c 3D images of BsaA localization in the pellicle biofilm formed by WT cells and in the adherent biofilm formed by the bsaA deletion mutant. Cells were grown at 25 °C for 2 days. Fixed biofilms were probed with anti-BsaA antibodies (red). Biofilm cells were stained with Syto9 (green).

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