Fig. 7: pilA2 and bsaS are necessary for heterogeneous sipW expression. | npj Biofilms and Microbiomes

Fig. 7: pilA2 and bsaS are necessary for heterogeneous sipW expression.

From: Temperature-regulated heterogeneous extracellular matrix gene expression defines biofilm morphology in Clostridium perfringens

Fig. 7

PsipW-evoglow expressing cells (green) are not located at the bottom. a, b Single-cell analysis of PsipW-evoglow expression in planktonic cells of ΔpilA2 or ΔbsaS. a Fluorescent microscopy images. Bar = 10 µm. b Quantitative analysis of the sipW promoter by FACS analysis. We analyzed 100,000 events for each sample. c 3D and orthometric images of the localization of sipW-expressing cells in pellicle biofilms of WT or ΔpilA2. Cells were grown for 1 day at 25 °C and stained with the membrane stain FM4-64 (2.5 µg ml−1) (red).

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