Fig. 3: Recombinant rEsp_N protein forms reversible aggregates in acidic phosphate–citrate buffer.
From: The biofilm-associated surface protein Esp of Enterococcus faecalis forms amyloid-like fibers
a 2 μM of purified rEsp_N protein was incubated in phosphate buffer solutions at different pH values. Aggregates started to be visible in buffers with pH 4 and 4.2 (indicated by a red arrow). b Reversion assay showed complete disassembly after phosphate–citrate buffer exchange from pH 4.2 to pH 7. c Scanning electron microscopy of rEsp_N aggregates. Scale bar represents 1 µm.
