Fig. 5: Binding of rEsp_N to amyloid-indicative dyes.
From: The biofilm-associated surface protein Esp of Enterococcus faecalis forms amyloid-like fibers
a Shift in CR absorbance spectrum upon binding of CR dye to 0.1 mg/ml rEsp_N at pH 7.0, pH 4.2, and pH-reverted samples to pH 7.0. Free CR absorbance spectrum is represented in black. b Increase in Th-T emission fluorescence upon binding to 0.1 mg/ml of rEsp_N at pH 7.0, pH 4.2, and pH-reverted samples to pH 7.0. c Fluorescence of rEsp_N aggregates stained with Proteostat dye. Samples were visualized using a ×100 oil immersion lens. Scale bar represents 10 µm. d Aggregation kinetics of rEsp_N at pH 4.2 (red) and at pH 7 (green), as monitored by changes in relative Th-T fluorescence signal. Free Th-T fluorescence is shown in black.
