Fig. 7: Exogenous complementation of Esp-negative strains with purified rEsp_N.
From: The biofilm-associated surface protein Esp of Enterococcus faecalis forms amyloid-like fibers
a Bacterial clumping of overnight cultures of esp-negative E. faecalis 23 and S. aureus Δbap grown under shaken conditions (200 rpm) at 37 °C in the presence of rEsp_N. Ø indicates no addition of rEsp_N to bacterial culture. b Inmunofluorescence showing surface localization of rEsp_N. Bacteria were fixed and labeled with anti-His antibodies and DAPI. Scale bars represent 3 µm. c Scanning electron microscopy and d transmission electron micrographs of negatively stained E. faecalis 23 incubated with (right panel) and without rEsp_N (left panel). Scale bar represents 500 nm.
