Fig. 6: Biofilm determination. | npj Biofilms and Microbiomes

Fig. 6: Biofilm determination.

From: IolR, a negative regulator of the myo-inositol metabolic pathway, inhibits cell autoaggregation and biofilm formation by downregulating RpmA in Aeromonas hydrophila

Fig. 6

Effect of EGTA (a) and proteinase K (b) on biofilm formation of the wild-type and ΔiolR mutant strain. The bacteria were cultured in LB medium supplemented EGTA at a range of concentrations from 0.01 to 10 mM or proteinase K at a range of concentrations from 20 to 80 mM for 18 h at 28 °C. Images were acquired with a camera. c CLSM images of biofilms of the wild-type and ΔiolR mutant strains. The viable cells exhibit red fluorescence. d Biofilm formation was observed by crystal violet staining and quantification analysis was performed by measuring the OD595 values. Error bars represent the standard deviations from at least three independent experiments. Statistical significance was determined using a two-tailed unpaired Student’s t test. ***P < 0.001.

Back to article page