Fig. 9: Transcription of flaA, flaB, flaC, and flaD genes.
From: Role of DegQ in differential stability of flagellin subunits in Vibrio vulnificus
a, b Gene clusters for flagellar assembly. V. vulnificus MO6-24/O genomes include four flagellin genes12, flaC (VVM06_02255) and flaD (VVM06_02252) at region I and flaB (VVM06_00808) and flaA (VVM06_00809) at region II. c–f Identification of transcription initiation sites (TIS) and promoters. Primer-extension experiments using a primer specific to each fla gene (Supplementary Table 2) revealed TIS and the promoters of flaA (c), flaB (d), flaC (e), and flaD (f). Lane C, T, A, and G represent the nucleotide sequencing ladders of the upstream region for each gene. An arrow denotes the cDNA band indicating TIS. TIS (designated with +1) and its promoter (underlined and aligned with the consensus sequences for RpoN or RpoF68,69) were indicated in nucleotide sequences upstream of the initiation codon (ATG) of each gene.
