Fig. 7: Reduction in fluorescence intensity is a result of glucose metabolism over time in biofilms of S. mutans.
From: Fluorescent nanosensors reveal dynamic pH gradients during biofilm formation
Biofilms of S. mutans NCTC 10832 were initially imaged after 48 h of static growth before either water or 1% glucose was added to the biofilm. Further images were taken every minute for 20 min, and then at 25 and 30 min. After 30 min, a 4.5 pH buffer was added and further images were taken every minute for 5 min. a 3D top view fluorescent microscope image of the fluorescence intensity changes over time. Green (OG-FAM), red (TAMRA) fluorescence with time point 0 min including DAPI-stained S. mutans cells shown in magenta. Scale bar represents 10 µm. b Fluorescence intensity of OG-FAM and TAMRA was measured using wide-field images in ImageJ and the normalised ratio is plotted against the time of incubation. Error bars represent standard error measured for different biofilm replicates, where n = 3.
