Fig. 1: Heterologous expression of metagenomic luxI homolog genes recovered from Ulu Pandan sludge microbial community in Escherichia coli Top10.
From: Functional metagenomic analysis of quorum sensing signaling in a nitrifying community
Detection of AHLs synthesized by different metagenomic luxI clones using AHL sensitive biosensors Chromobacterium violaceum CV026 and Agrobacterium tumefaciens A136 (a) or E. coli JBA525 (b). Distribution of metagenomic luxI clones according to their capacity to activate different AHL-sensitive biosensors (c). Chemical characterization and quantification of AHL species using HPLC–MSMS (d). The relative quantity of different AHL species synthesized by the individual clone is indicated by the bubble size. US: Ulu Pandan Synthase (Metagenomic luxI clone); +VE: positive control; −VE: negative control. Data are presented as mean ± SD.
