Fig. 4: Regulation of NspR1 on the predicted lux-box containing promoters with and without the co-expression of Nitrospira sigma factors. | npj Biofilms and Microbiomes

Fig. 4: Regulation of NspR1 on the predicted lux-box containing promoters with and without the co-expression of Nitrospira sigma factors.

From: Functional metagenomic analysis of quorum sensing signaling in a nitrifying community

Fig. 4

The screening was performed in E. coli TOP10 transformed with pPROBE-gfp(ASV) containing the predicted lux-box containing promoters (Nspbox) and pUCP22-NspR1 or pUCP22-NspR1 carrying the Nitrospira sigma factor gene. Relative fold induction was calculated by measuring the fold induction of GFP signals of the induced sample (added with 1 mM C8-HSL) to uninduced control (added with DMSO). Data are presented as mean ± SD.

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