Fig. 5: Purification and DNA-binding of NspR1.
From: Functional metagenomic analysis of quorum sensing signaling in a nitrifying community
Purification of soluble NspR1 using Ni-NTA column (a). Soluble 6xHis-tagged NspR1 was obtained by overexpressing the protein in the presence of 5 μM C8-HSL at 17 °C for 16 h. DNA-binding of purified NspR1 to the DNA probe containing Nspbox1 sequence, Nsp104 and scramble control Scr104, in the presence and absence of E. coli RNA polymerase holoenzyme (b). The EMSA gel for both Scr104 and Nsp104 samples were derived from the same experiment and then they were processed in parallel.
