Fig. 2: Phenotypic determination of the evolved variants.

From left to right: colony morphologies, Congo red uptake, swarming motility on EPS and Trb medium, swimming motility, cellular morphologies in LB liquid, biofilm formation in LB and EPS medium in shaken cultures. Anc, FS, and N indicate the ancestor, FS, and N morph variants, respectively. Scale bars in colony and surface motility images indicate 5 mm and that in single cellular morphologies indicates 10 µm across all groups. Arrows indicate the formed biofilm in the LB shaken samples.