Fig. 2: Polymicrobial oral infection is reduced by nisin or nisin-producing probiotic treatment. | npj Biofilms and Microbiomes

Fig. 2: Polymicrobial oral infection is reduced by nisin or nisin-producing probiotic treatment.

From: Nisin probiotic prevents inflammatory bone loss while promoting reparative proliferation and a healthy microbiome

Fig. 2

Oral swab samples were collected at 8 weeks after polymicrobial infection. DNA was isolated and purified from the swab samples of eight groups (Control, Infection, Nisin (H), L. lactis, Non-nisin L. lactis, Infection + nisin (H), Infection + L. lactis and Infection + Non-nisin L. lactis; n = 6 mice per group). The total bacteria were quantified by standard real-time PCR using primers corresponding to 16S ribosomal RNA. A The data are shown as a percentage of each pathogen (P. gingivalis, T. denticola, T. forsythia, or F. nucleatum) among total bacteria. Data represent the means ± standard deviation from six mice per group.Statistical significance was determined using an ANOVA followed by a Tukey’s test. The difference in variance with a p-value of <0.05 was considered significant. (a) The difference in percentage of the pathogen was significant (p < 0.001) compared to the Control group. (b) The difference in percentage of the pathogen was significant (p < 0.01) compared to the Infection group. *, the difference in percentage of the pathogen between the two groups was significant (p < 0.05). B The table demonstrates the number of detected bacteria and detection frequency (%) of periodontal pathogens in each swab from each mouse relative to the number of collected samples.

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