Fig. 1: Construction of engineered Bap with fusion domains.
From: Bacterial biofilm functionalization through Bap amyloid engineering
a Collection of tags fused to the end of the amyloid domain B of Bap: His-tag (6 aa); SpyTag (13 aa); Mefp3 (48 aa); MT1 (61 aa); SNAP-tag (181 aa); mCherry (235 aa). b S. aureus expressing engineered Bap grow in suspension when they are cultured in LB media (upper panel) or form bacterial clumps in LB with glucose 0.5% (w/v) (LB-glu) overnight cultures grown under shaken conditions (200 rpm) at 37 °C (bottom panel). c Colony morphologies of S. aureus expressing engineered Bap on Congo red agar after 24 h of incubation. d Biofilms formed by S. aureus expressing engineered Bap were stained with crystal violet. For biofilm formation, bacteria were cultured in LB or LB-glu overnight at 37 °C in microtiter plates under static conditions. e Biofilms formed in LB (grey bars) or LB-glu (white bars) were quantified by solubilizing the crystal violet with alcohol-acetone and determining the absorbance at 595 nm. The error bars represent standard deviations of 3 repetitions. Statistical significance differences were determined using non-parametric one-tail Mann Whitney test: *P < 0.05.
