Fig. 6: Real time immobilization of epitopes under flow culture conditions using SpyTag/SpyCatcher system.
From: Bacterial biofilm functionalization through Bap amyloid engineering
a Schematic diagram of the experiment. S. aureus Bap-Spy was grown in CellAsic microfluidic plates at 37 °C with a continuous flow for 300 min. Then, rCatcher-GFP (left column) and rGFP (right column) were added and the flow was maintained during 2 h with the recombinant proteins. Next, channels were washed with PBS during 90 min to remove the background fluorescence. b Images of GFP fluorescence and the differential interference contrast (DIC) at different times are shown. S. aureus Bap-Spy was grown in microfluidic plates and were incubated with rCatcher-GFP (left column) and rGFP (right column). Images were recorded every 30 min in separate fields to avoid photobleaching. Scale bar of panels represents 5 μm.
