Fig. 7: Recombinant rBapB-Spy protein forms aggregates under acidic conditions that can be functionalized with rCatcher-GFP.
From: Bacterial biofilm functionalization through Bap amyloid engineering
Schematic illustration showing a rBapB-Spy and b functionalized fibers after exogenous complementation with rBapB-Spy. c rBapB-Spy aggregates under acidic conditions in a similar way of rBapB. 2 μM of purified recombinant proteins (rBapB-Spy and rBapB) were incubated in phosphate-citrate buffer at pH 4.5 and pH 7. Aggregates were only visible at acid pH. d rBapB-Spy and rBapB aggregation kinetics were monitored by following the changes in relative ThT fluorescence emission intensity. e Immunofluorescence showing rBapB-Spy aggregates probed with purified recombinant rCatcher-GFP and purified rGFP alone (used as a control). The fluorescence of GFP and the differential interference contrast (DIC) images are shown. Scale bar of panels represents 5 μm.
