Fig. 8: Functionalization of bacteria through exogenous complementation with rBapB-Spy.
From: Bacterial biofilm functionalization through Bap amyloid engineering
a Exogenous complementation of biofilm negative bacteria S. aureus Δbap, E. faecalis 23 and L. monocytogenes EGD strains with rBapB-Spy coupled to purified recombinant rCatcher-GFP and purified rGFP alone (used as a control). b Fluorescence under uv-light of the rings formed by S. aureus Δbap, E. faecalis 23 and L. monocytogenes EGD cells when they are incubated with rBapB-Spy coupled to purified recombinant rCatcher-GFP and purified rGFP. c The fluorescence of GFP and Hoechst, the combination of both signals (merge panels) and the differential interference contrast (DIC) images are shown. Scale bar of panels represents 5 μm.
