Table 1 Examples of currently used standards for biofilm testing.

AATCC TM100 -2019

Test method for antibacterial finishes on textile materials

https://microchemlab.com/test/aatcc-100-antimicrobial-fabric-test/

ASTM E2196-17

Standard test method for quantification of Pseudomonas aeruginosa biofilm grown with medium shear and continuous flow using rotating disk reactor

https://www.astm.org/e2196-17.html

ASTM E2315-16

Standard guide for assessment of antimicrobial activity using a time-kill procedure

https://www.astm.org/e2315-16.html

ASTM E2562-17

Standard test method for quantification of Pseudomonas aeruginosa biofilm grown with high shear and continuous flow using CDC (Center for Disease Control) biofilm reactor

https://www.astm.org/e2562-17.html

ASTM E2647-20

Standard test method for quantification of Pseudomonas aeruginosa biofilm grown using drip flow biofilm reactor with low shear and continuous flow

https://www.astm.org/e2647-20.html

ASTM E2799-17

Standard test method for testing disinfectant efficacy against Pseudomonas aeruginosa biofilm using the MBEC Assay

https://www.astm.org/e2799-17.html

ASTM E2871-21

Standard test method for determining disinfectant efficacy against biofilm grown in the CDC biofilm reactor using the single tube method

https://www.astm.org/e2871-21.html

ASTM E3161-21

Standard practice for preparing a Pseudomonas aeruginosa or Staphylococcus aureus biofilm using the CDC biofilm reactor

https://www.astm.org/e3161-21.html

ASTM E3180-18

Standard test method for quantification of a Bacillus subtilis biofilm comprised of vegetative cells and spores grown using the colony biofilm model

https://www.astm.org/e3180-18.html

ASTM E3321-21

Standard test method for intraluminal catheter model used to evaluate antimicrobial urinary catheters for prevention of Escherichia coli biofilm growth

https://www.astm.org/e3321-21.html

ASTM E3151-18

Standard test method for determining antimicrobial activity and biofilm resistance properties of tube, yarn, or fiber specimens

https://www.astm.org/e3151-18.html

ASTM E645-18

Standard practice for evaluation of microbicides used in cooling water systems

https://www.astm.org/e0645-18.html

ISO/DIS 4768

Measurement method of anti-biofilm activity on plastic and other non-porous surfaces

https://www.iso.org/standard/80309.html

ISO 16954:2015

Test methods for evaluating the efficiency of treatment methods intended to prevent biofilm formation or removal in dental unit procedural water delivery systems under laboratory conditions.

https://www.iso.org/standard/58009.html

ISO 11731:2017

Water quality - Enumeration of Legionella

https://www.iso.org/standard/61782.html