Fig. 1: VC1826 is a mannose PTS transporter and forms an operon with VC1827 encoding mannose 6-phosphate isomerase. | npj Biofilms and Microbiomes

Fig. 1: VC1826 is a mannose PTS transporter and forms an operon with VC1827 encoding mannose 6-phosphate isomerase.

From: A mannose-sensing AraC-type transcriptional activator regulates cell–cell aggregation of Vibrio cholerae

Fig. 1

a Relative mRNA expression of VC1826 in the wild-type V. cholerae strain grown on various carbohydrates: NAG, N-acetylglucosamine; NAM, N-acetylmannosamine. The mRNA expression levels are shown as relative values (log2 scale) to that in cells grown on glycerol. The means and standard deviations of three independent measurements are shown. b Growth curves of the WT (black squares) and VC1826 deletion mutant (white squares) in M9 medium supplemented with 0.2% glucose, fructose, and mannose. Growth was measured by recording the absorbance at 600 nm using a multimode microplate reader (TECAN). The means of three independent measurements are shown. c Analysis of co-transcription of VC1826 with VC1827 using reverse transcription (RT)-PCR. Double-headed arrows with numbers indicate RT-PCR products shown below. RT-PCR products (lanes C) were electrophoresed on an 1% agarose gel with the corresponding PCRs without reverse transcriptase (lanes R); PCR products were amplified using genomic DNA (lanes D) as a template to verify primer specificity. Lane M refers to the DNA size marker (ThermoFisher Scientific).

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