Fig. 6: SsnA disperses pre-established S. mutans biofilm in a pH dependent manner. | npj Biofilms and Microbiomes

Fig. 6: SsnA disperses pre-established S. mutans biofilm in a pH dependent manner.

From: Interspecies competition in oral biofilms mediated by Streptococcus gordonii extracellular deoxyribonuclease SsnA

Fig. 6

a Confocal laser scanning microscopy of pre-established 20 h biofilms of S. mutans treated with PBS (control) or 5 µg/mL SsnA for 1 h at 37 °C. Propidium iodide (20 μM) was used to visualise the cells. Scale bars are 30 µm (left panels) or 20 µm (right panels). b Biomass of pre-established S. mutans biofilms treated with DNase I (5 µg/mL) or SsnA (5 µg/mL) at pH 4, 5, 6, and 7. Crystal violet assay was used for quantification. Data points are shown for 3 independent repeats, bars represent the mean and SD is indicated.

Back to article page