Fig. 7: SsnA prevents accumulation of oral microcosm biofilms grown under flow of natural human saliva.

a Visualisation of oral microcosm biofilms developed in the channel of BioFlux dual inlet microfluidic system with saliva containing SsnA (5 µg/mL) feeding the bottom half of the channel. Microcosm biofilms were grown for 20 h. Yoyo-1 (2.4 nM) was used to stain eDNA within the biofilm. b Quantification of eDNA accumulation for the SsnA-containing and SsnA-free section of the growth channel over 20 h. Images were taken every 10 min and eDNA amounts were determined using GrayValue within FIJI software. c Visualisation of microcosm biofilms grown in 0.3% sucrose-supplemented natural saliva. SsnA (5 µg/mL) was included in the reservoir feeding the bottom half of the channel. Yoyo-1 (2.4 nM) was included for eDNA staining. d Quantification of eDNA accumulation for the SsnA-containing and SsnA-free section of the growth channel over 20 h of growth in saliva supplemented with 0.3% sucrose. Images were taken every 10 min and eDNA amounts were determined using GrayValue within FIJI software. Mean and SE from 3 independent repeats are shown. Scale bars are 50 µm.