Fig. 4: C. albicans assembly of amyloid structures upon adhesion requires Pga59. | npj Biofilms and Microbiomes

Fig. 4: C. albicans assembly of amyloid structures upon adhesion requires Pga59.

From: The Pga59 cell wall protein is an amyloid forming protein involved in adhesion and biofilm establishment in the pathogenic yeast Candida albicans

Fig. 4: C. albicans assembly of amyloid structures upon adhesion requires Pga59.

a Amyloid structures formation in C. albicans was monitored by using ThT staining. WT, pga59Δ/pga59Δ, PGA59/pga59Δ and pga59V32,33N/pga59Δ strains were cultured in a YPD medium. Adhesion was triggered by adding magnetic beads to the culture medium. Fungal cells without magnetic beads were used as a negative control (no adhesion, left panels). The ThT signal was recorded in the yellow channel while cells were observed by Nomarski (with a 20X objective). Scale bar: 25 μm. b Amyloid assembly was monitored with ThT staining (yellow channel) at higher magnification (with a 100X objective). Amyloid structures found in the cell wall are indicated with white arrows. Scale bar: 3 μm.

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